Question: How Do You Dilute Beta Mercaptoethanol?

How much mercaptoethanol is in a sample buffer?

Add 50 µl of β-mercaptoethanol per 950 µl of sample buffer for a final concentration of 5% β-mercaptoethanol, 710 mM.

As an alternative, dithiothreitol (DTT or Cleland’s reagent) may be used at a final concentration of 350 mM (54 mg/ml).

Dilute 1 part sample with 1 part Laemmli sample buffer..

Is BME a reducing agent?

Pure liquid (14 M), beta-mercaptoethanol (BME, 2BME, 2-ME, b-mer, CAS 60-24-2) is a thiol reducing agent for cleaving protein disulfide bonds (cystine).

How do you neutralize beta mercaptoethanol?

BME odor can be neutralized using standard household bleach. Bleach acts as an oxidizer and converts the thiol group of beta mercaptoethanol into a sulfonic acid derivative which eliminates the natural gas odor. Be sure to absorb any excess BME liquid with an inert absorbent prior to odor decontamination with bleach.

How dangerous is beta mercaptoethanol?

Beta-Mercaptoethanol is considered a “severe” poison, causing “irritation to the nasal passageways and respiratory tract upon inhalation, vomiting and stomach pain through ingestion, and potentially fatal absorption if it contacts the skin”.

Is beta mercaptoethanol flammable?

Not flammable or combustible. Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide.

How does 2 mercaptoethanol work?

2-Mercaptoethanol is used in some RNA isolation procedures to eliminate ribonuclease released during cell lysis. Numerous disulfide bonds make ribonucleases very stable enzymes, so 2-mercaptoethanol is used to reduce these disulfide bonds and irreversibly denature the proteins.

How do you store beta mercaptoethanol?

Store in a tightly closed container in a cool, dry area that is well ventilated, such as a vented cabinet. Do not store on shelf. Store in a secondary container by itself. Compounds incompatible with Beta Mercaptoethanol are oxidizers, acids, metals, and ignition sources.

What is the molarity of 2 mercaptoethanol?

14.3 MPropertiesvapor density2.69 (vs air)vapor pressure1 mmHg ( 20 °C)assay98%expl. lim.18 %concentration14.3 M (pure liquid)6 more rows

Is BME toxic?

BME can be toxic if ingested, and fatal if inhaled or absorbed through the skin. Vapors can irritate the eyes, mucous membranes, and respiratory tract. Symptoms of inhalation exposure may include coughing, sore throat, and/or shortness of breath. … BME is combustible as a liquid or vapor!

Is beta mercaptoethanol the same as 2-mercaptoethanol?

Gibco™ 2-Mercaptoethanol (also known as beta-mercaptoethanol or BME) is a potent reducing agent used in cell culture media to prevent toxic levels of oxygen radicals.

What does β mercaptoethanol do?

Beta-mercaptoethanol (ß-ME) is a reducing agent that will irreversibly denature RNases by reducing disulfide bonds and destroying the native conformation required for enzyme functionality.

Is beta mercaptoethanol carcinogenic?

Causes skin irritation and may be absorbed in the body in toxic quantities. Vapors irritate the eyes with redness and pain. Splashes may cause severe irritation. Substance is neither a known nor an anticipated carcinogen.

Why is beta-mercaptoethanol used in SDS PAGE?

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE.